603 papers
This study demonstrates that the innate immunity receptors CD14 and TLR4 act as tissue-specific co-receptors that, through MyD88-dependent kinase signaling and circadian replenishment, collaborate with other receptors like MerTK and CD36 to regulate the daily peak of photoreceptor outer segment phagocytosis in retinal pigment epithelium cells.
Using optical tweezers, this study demonstrates that direct tensile force applied to the N-terminus of the adhesion GPCR Adgrl3 directly activates G protein signaling in living cells through a direction-specific, tethered agonist-dependent mechanism involving GAIN-domain conformational changes.
By employing ribosome profiling to map the translatome of senescent human fibroblasts, this study reveals that early senescence is characterized by extensive post-transcriptional regulation that suppresses inflammatory SASP factors and cell cycle genes while activating retrotransposon translation, thereby establishing translational control as a critical layer in shaping the senescent phenotype.
This study reveals that the Parkinson's disease-associated LRRK2 G2019S mutation induces reactive oxygen species-dependent nuclear DNA damage, leading to unresolved base excision repair and cytotoxic PARP1 hyperactivation that renders cells vulnerable to PARP-trapping inhibitors.
This study demonstrates that optogenetic recruitment of deregulated PLC-γ1 mutants to the membrane is sufficient to trigger lipid hydrolysis and spatially direct cell motility, revealing that phosphorylation at Tyr783 is a marker of dysregulated autoinhibition rather than a direct proxy for enzymatic activity.
This paper proposes and demonstrates a mathematical framework that utilizes a minimization problem to quantify insulin-stimulated glucose uptake per adipocyte based on cell size, showing that incorporating size-dependent uptake improves the agreement between model predictions and experimental data.
This study demonstrates that S100A4 silencing in JAR choriocarcinoma cells specifically inhibits proliferation and migration without inducing apoptosis or altering invasion, while triggering compensatory signaling rewiring involving elevated IRS1/PI3K and suppressed Akt1.
This study demonstrates that co-treatment with a 15-PGDH inhibitor, which boosts PGE2 levels, counteracts the muscle regenerative deficits and impaired stem cell function caused by semaglutide-induced weight loss, thereby preserving skeletal muscle mass and strength.
In differentiated HL-60 neutrophil-like cells, BzATP-activated P2X7 receptor signaling triggers a PI3K/AKT-MRP1-dependent pathway that depletes intracellular glutathione to activate nSMase, thereby driving ceramide-mediated exosome biogenesis as a mechanism for translating inflammatory cues into vesicle communication.
This study demonstrates that cardiac-specific deletion of Protein Phosphatase 2A catalytic subunit (PP2Ac) disrupts mitochondrial dynamics by causing ERK2 hyperphosphorylation, which upregulates Fis1 and Drp1-mediated fission, leading to excessive mitophagy and hypertrophic cardiomyopathy.
This study demonstrates that MIRO1 is a critical regulator of vascular smooth muscle cell proliferation and neointima formation by maintaining mitochondrial cristae integrity for ATP synthesis and utilizing Ca2+-dependent EF hands to position mitochondria, thereby linking mitochondrial motility and energy production to vascular remodeling.
The paper introduces SPACE, a cost-effective, high-throughput spatial CRISPR screening platform that integrates whole-transcriptome and multiplexed protein profiling at subcellular resolution within 3D tissue models to uncover spatially resolved regulatory mechanisms and ligand-receptor interactions invisible to dissociation-based methods.
The study reveals that the contractile peri-nuclear actomyosin network (PANEM) repositions chromosomes from unfavorable peripheral and polar locations immediately after nuclear envelope breakdown to facilitate initial kinetochore-microtubule interactions and ensure high-fidelity chromosome segregation.
By integrating large-scale proteomics, evolutionary conservation, machine learning, and chemical genomics, this study establishes a comprehensive human reference ubiquitinome and identifies a functional importance score that distinguishes site-specific ubiquitination roles in signaling versus degradation, ultimately revealing how specific modifications like K320 in ELAVL1 regulate critical cellular functions.
This study establishes the zebrafish otic vesicle and mouse epididymis as optimized in vivo models to elucidate the conserved and tissue-specific mechanisms of columnar epithelial cell division, revealing that dynein-driven apical nuclear migration and actomyosin-mediated basolateral constriction are critical for proper mitotic rounding and daughter cell integration.
This study demonstrates that boldine, an alkaloid from *Peumus boldus*, prevents diabetes-induced skeletal muscle dysfunction, lipid accumulation, and inflammation in mice and human myoblasts by inhibiting large-pore channel activity and subsequently suppressing calcium-dependent inflammasome activation and adipogenic pathways.
This study demonstrates that the ER-vacuole tether Mdm1 spatially coordinates sphingolipid metabolism to enable the adaptive endocytic remodeling of the methionine transporter Mup1, thereby maintaining amino acid homeostasis and extending chronological lifespan in budding yeast.
This study reveals that, contrary to the absence of global poleward flux in *C. elegans* zygotes, a localized flux occurs specifically in kinetochore microtubules during metaphase and is driven by the kinesin-12 motor KLP-18 sliding these microtubules along spindle microtubules rather than through poleward treadmilling.
This study reveals that the anti-cancer drug 5-aza-cytidine promotes the usage of terminal polyadenylation sites to generate full-length mRNA transcripts in tumor cells by upregulating anti-termination factors and downregulating early termination enhancers, thereby shifting the transcriptome from shortened to complete isoforms.
This paper introduces an optimized enzymatic digestion and serum-free culture method that enables long-term ex ovo development of *C. elegans* embryos, thereby overcoming eggshell permeability barriers to allow precise pharmacological manipulation and study of developmental processes through adulthood.